رزومه دکتر قلاسی رزومه دکتر قلاسی

Curriculum Vitae

Marzieh Ghollasi

Date of Birth: 28/7/1979

Address: Department of cell and molecular biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran, P.O Box: 31979-37551

Cell: +989128596570

Email: ghollasi@khu.ac.ir

Education 

Tarbait Modares University, Tehran, Iran        Oct. 2004- Jan.2009

Ph.D., Biochemistry

GPA  18.67/20

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Alzahra University, Tehran, Iran                      Feb.2001- Sep.2004

Master of Science, Biochemistry

Dissertation Title: "Comparison of catalytic activity in dsDNA and ssDNA" by Dr. Bijan Farzami, Zahra Bathaie, Behzad Lame-Rad

GPA  19.25/20

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Tehran University , Tehran, Iran                      Oct. 1997-Sep.2001

Bachelor of Science, Cellular and molecular Biology

GPA 16.82/20

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Awards and Honors:

2010, Distinguished young scientist in 9th Iranian conference of Biophysical chemistry, Tehran, Iran.

2010, Academic excellent award for phD thesis at Tarbiat Modares University, Tehran, Iran.

2004, First rank in the phD exam at Tarbiat Modares University, Tehran, Iran.

2004, Top student during M.Sc, Alzahra University, Tehran, Iran. 

 

Research Interests

Structure activity relationship of enzymes

Immobilization of enzymes on nanostructures

Protein engineering

Stem cells biology

Nano-biotechnology

 

Teaching Experiences

Year: 2012- Till now

Department of cellular and molecular biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran

Assistant Professor

Year: 2010- Till now

Department of biology, Faculty of Science, Shahed University, Tehran, Iran

 

Skills·

· Designing of primers for mutation
 
· Molecular skills such as Primer designing, Cloning of gene in cloning and expressing vectors, transformation of vector into E.coli by chemical and electroporation methods
 
· Purification of proteins by chromatography
 
· Working with FPLC
 
· Working with HPLC as analytical and preparative instrument
 
· Enzymatic studying such as assay of enzymatic activity, defining thermo stability and kinetic parameters of an enzyme,
 
· Protein Structure analysis by Swiss PDB viewer
 
· Phylogenetic Analysis, Pair Wise and Multiple Sequence Alignment
 
 

Publications

Published Journal Article:

1. Ali Salimi, Samad Nadri, Marzieh Ghollasi, Khosro Khajeh, Masoud Soleimani. Comparison of different protocols for neural differentiation of human induced pluripotent stem cells. Molecular Biology Reports 2014, 41(3):1713-1721

2. Marzieh Ghollasi, Khosro Khajeh, Maryam Ghanbari Safari. Improvement of thermal stability of a mutagenised α-amylase by manipulation of the calcium-binding site. Enzyme and microbial technology 2013; 53(6-7):406-13.

3. Ali Salimi, Sirous Ghobadi, Marzieh Ghollasi, Fatemeh Yousefi, Sara Daneshjou, Khosro Khajeh. Investigations on Possible Roles of C-Terminal Propeptide of a Ca-Independent α-Amylase from Bacillus. Journal of Microbiology and Biotechnology 2012, 22(8), 1077–1083

4. Marzieh Ghollasi, Khosro Khajeh, Hossein Naderi-Manesh, Atiyeh Ghasemi. Engineering of a Bacillus α-amylase with improved thermostability and calcium independency. Applied Biochemistry and Biotechnology 2010: 162:444–459

5. Shekufeh Zareian, Khosro Khajeh, Marzieh Ghollasi, Nasrin Mollania, Bijan Ranjbar. Purification and characterization of a new and distinct amylopullulanase that converts pullulan to glucose, maltose, and maltotriose and starch to glucose and maltose. Enzyme and Microbial Technology 2010, 46: 57–63

6. Marzieh Ghollasi, Khosro Khajeh, Nasrin Mollania, Shekufeh Zareian & Hossein Naderi-Manesh. An investigation on acarbose inhibition and the number of active sites in an amylopullulanase (L14-APU) from an Iranian Bacillus sp. Biologia 2008, 63/6: 1051-1056

7. M. Ghollasi, B. Farzami, S.Z. Bathaie and B.L.Rad. Enzymatic- Like Properties of dsDNA and Its Comparison with ssDNA. Journal of Science, Islamic Republic of Iran. 2005, 16(1): 11-14

 

Refereed Conference Papers

1. Bahram Amiri, Marzieh Ghollasi, Mehdi Kamali, Ali Salimi, Ali Ardeshiry lajimi. "Study of osteoblast differentiation of mesenchymal stem cells on polyethersulfone- polyethylene glycol composite surface modificated nanofibers loading with Zn2Sio4 bioceramic nanoparticles". Asian nano forum conference, Iran. 2015

2. Ali Salimi, Samad Nadri, Marzieh Ghollasi, Khosro Khajeh, Masoud Soleimani. "Neural differentiation of human induced pluripotent stem cells with high efficiency". 5th annual research congress of Baqiyatallah university, Iran. 2010

3. Ali Salimi, Khosro Khajeh, Sirous Ghobadi, Marzieh Ghollasi. "Structural Studies of Two Forms of Alpha Amylase from Bacillus sp. KR-8104". 16th international &4th national congress on biology, Iran. 2010

4. Marzieh Ghollasi, Khosro Khajeh. "Thermostability Improvement of "BMW-amylase" from an Iranian Bacillus sp". 16th international &4th national congress on biology, Iran. 2010

5. Marzieh Ghollasi, Khosro Khajeh, Ali Salimi. "Characterization of a mutagenised thermal- resistant α-amylase from Bacillus megaterium WHO". 9th National congress on Biochemistry and Biophysics, Iran.2009

6. Ali Salimi, Khosro Khajeh, Sirous Ghobadi, Marzieh Ghollasi and Jahan AliKhajeh. "Cloning, Sequencing, Expression and Characterization of a Ca2+-independent α-amylase from Bacillus sp. KRA2: emphasis on the identification of C-terminal propeptide".10th National congress on Biochemistry & 3th internatiol congress on molecular biology Iran. 2009

7. Ali Salimi, Khosro Khajeh, Sirous Ghobadi, Marzieh Ghollasi and Jahan AliKhajeh. "Characterization of the C-terminal propeptide by cloning, sequencing and expression of a Ca2+ independent α-amylase from Bacillus sp. KRA2". 14th European Congress on Biotechnology. 2009

8. Marzieh Ghollasi, Khosro Khajeh, Hossein Naderi-Manesh. "Engineering of a Bacillus α-amylase whose stability is independent of calcium". 14th European Congress on Biotechnology. 2009

9. Ali Salimi, Jahan AliKhajeh, Mohhamad Pazhang,  Marzieh Ghollasi, Sirous Ghobadi & Khosro Khajeh "Alpha Amylase from an Iranian Bacillus sp". 15th National & 3th internatiol congress on biology, 2008

10. Maryam Ghanbari Safari, Khosro Khajeh, Marzieh Ghollasi, Hossein Naderi-manesh. "A novel extracellular α-amylase from a new isolated Bacillus sp. WHO: cloning, purification and biochemical characterization". 10th national Genetics Conference. 2008

11. Shekufeh Zareian, Khosro Khajeh, Marzieh Ghollasi, Bijan Ranjbar. "Purification and characterization of a novel pullulanase which converts pullulan and starch to glucose and maltose". 33rd FEBS Congress & 11th IUBMB Conference. 2008

12. Maryam Ghanbari Safari, Khosro Khajeh, Marzieh Ghollasi, Hossein Naderi-manesh. "A novel extracellular α-amylase from a new isolated Bacillus sp. WHO: cloning, purification and biochemical characterization" 33rd FEBS Congress & 11th IUBMB Conference 2008

13. Marzieh Ghollasi, Khosro Khajeh, Nasrin mollania, Shekufeh Zareian. "Isolation, purification and biochemical analysis of a new pullulanase from Geobacillus stearothermophilus". The 3 rd Symposium on the Alpha-Amylase Family 2008

14. Shekufeh Zareian, Khosro Khajeh, Reza H.Sajedi, Bijan Ranjbar & Marzieh Ghollasi. "Screening, Isolation, Purification and characterization of pullulanase from a thermophilic strain of an hot spring". 9th national & 2th international congress on biochemistry and molecular biology. 2007

15. Nasrin mollania, Khosro Khajeh, Mehdi Naderi-manesh, Marzieh Ghollasi. "Isolation, purification, biochemical characterization and cloning of a new extracellular α-amylase from Geobacillus thermodenitroficans LH8 which is insensitive to phytic acid". The 3 rd Symposium on the Alpha-Amylase Family 2007

16. Shekufeh Zareian, Marzieh Ghollasi, Khosro Khajeh, Saman Hosseinkhani, Bijan Ranjbar." Isolation, purification and biochemical analysis of a new pullulanase from Geobacillus stearothermophilus". The 3 rd Symposium on the Alpha-Amylase Family 2007.

17. M. Ghollasi, B. Farzami, S.Z. Bathaie and B.L.Rad. "Enzymatic- Like Properties of dsDNA and Its Comparison with ssDNA". 6th national congress on biochemistry & biophysic, Iran. 2005

 

Teaching Experiences

· Enzymology (for M.Sc students of Biotechnology)

· Radiation biology (for B.Sc students of cellular and molecular biology)

· Biochemistry (for B.Sc students of biology)

· Biochemistry of proteins and nucleic acids (for M.Sc students of Biochemistry)