Curriculum Vitae

Marzieh Ghollasi

Date of Birth: 28/7/1979

Address: Department of cell and molecular biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran, P.O Box: 31979-37551

Cell: +989128596570

Email: ghollasi@khu.ac.ir

Education  

Tarbait Modares University, Tehran, Iran        Oct. 2004- Jan.2009

   Ph.D., Biochemistry

 GPA  18.67/20

Alzahra University, Tehran, Iran                      Feb.2001- Sep.2004

   Master of Science, Biochemistry

Dissertation Title: "Comparison of catalytic activity in dsDNA and ssDNA" by Dr. Bijan Farzami, Zahra Bathaie, Behzad Lame-Rad

GPA  19.25/20

Tehran University , Tehran, Iran                      Oct. 1997-Sep.2001

Bachelor of Science, Cellular and molecular Biology

GPA 16.82/20

Awards and Honors:

- 2010, Distinguished young scientist in 9th Iranian conference of Biophysical chemistry, Tehran, Iran.

- 2010, Academic excellent award for phD thesis at Tarbiat Modares University, Tehran, Iran.

-2004, First rank in the phD exam at Tarbiat Modares University, Tehran, Iran.

- 2004, Top student during M.Sc, Alzahra University, Tehran, Iran.  

Research Interests

Structure activity relationship of enzymes

Immobilization of enzymes on nanostructures

protein engineering

Stem cells biology

Nanobiotechnology

Teaching Experiences

Year: 2012- Till now

Department of cellular and molecular biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran

Assistant Professor

Year: 2010- Till now

Department of biology, Faculty of Science, Shahed University, Tehran, Iran

Skills

Ø  Designing of primers for mutation

Ø  Molecular skills such as Primer designing, Cloning of gene in cloning and expressing vectors, transformation of vector into E.coli by chemical and electroporation methods

Ø  Purification of proteins by chromatography

Ø  Working with FPLC

Ø  Working with HPLC as analytical and preparative instrument

Ø  Enzymatic studying such as assay of enzymatic activity, defining thermostability and kinetic parameters of an enzyme,

Ø  Protein Structure analysis by Swiss PDB viewer

Ø  Phylogenetic Analysis, Pair Wise and Multiple Sequence Alignment

Publications

Published Journal Article:

1.       Ali Salimi, Samad Nadri, Marzieh Ghollasi, Khosro Khajeh, Masoud Soleimani. Comparison of different protocols for neural differentiation of human induced pluripotent stem cells. Molecular Biology Reports 2014, 41(3):1713-1721

2.       Marzieh Ghollasi, Khosro Khajeh, Maryam Ghanbari Safari. Improvement of thermal stability of a mutagenised α-amylase by manipulation of the calcium-binding site. Enzyme and microbial technology 2013; 53(6-7):406-13.

3.       Ali Salimi, Sirous Ghobadi, Marzieh Ghollasi, Fatemeh Yousefi, Sara Daneshjou, Khosro Khajeh. Investigations on Possible Roles of C-Terminal Propeptide of a Ca-Independent α-Amylase from Bacillus. Journal of Microbiology and Biotechnology 2012, 22(8), 1077–1083

4.       Marzieh Ghollasi, Khosro Khajeh, Hossein Naderi-Manesh, Atiyeh Ghasemi. Engineering of a Bacillus α-amylase with improved thermostability and calcium independency. Applied Biochemistry and Biotechnology 2010: 162:444–459

5.       Shekufeh Zareian, Khosro Khajeh, Marzieh Ghollasi, Nasrin Mollania, Bijan Ranjbar. Purification and characterization of a new and distinct amylopullulanase that converts pullulan to glucose, maltose, and maltotriose and starch to glucose and maltose. Enzyme and Microbial Technology 2010, 46: 57–63

6.       Marzieh Ghollasi, Khosro Khajeh, Nasrin Mollania, Shekufeh Zareian & Hossein Naderi-Manesh. An investigation on acarbose inhibition and the number of active sites in an amylopullulanase (L14-APU) from an Iranian Bacillus sp. Biologia 2008, 63/6: 1051-1056

7.       M. Ghollasi, B. Farzami, S.Z. Bathaie and B.L.Rad. Enzymatic- Like Properties of dsDNA and Its Comparison with ssDNA. Journal of Science, Islamic Republic of Iran. 2005, 16(1): 11-14

Refereed Conference Papers

1. Bahram Amiri, Marzieh Ghollasi, Mehdi Kamali, Ali Salimi, Ali Ardeshiry lajimi. “Study of osteoblast differentiation of mesenchymal stem cells on polyethersulfone- polyethylene glycol composite surface modificated nanofibers loading with Zn₂Sio₄ bioceramic nanoparticles”. Asian nano forum conference, Iran. 2015
2. Ali Salimi, Samad Nadri, Marzieh Ghollasi, Khosro Khajeh, Masoud Soleimani. “Neural differentiation of human induced pluripotent stem cells with high efficiency”. 5^th annual research congress of Baqiyatallah university, Iran. 2010
3. Ali Salimi, Khosro Khajeh, Sirous Ghobadi, Marzieh Ghollasi. “Structural Studies of Two Forms of Alpha Amylase from Bacillus sp. KR-8104”. 16^th international &4^th national congress on biology, Iran. 2010
4. Marzieh Ghollasi, Khosro Khajeh. “Thermostability Improvement of “BMW-amylase” from an Iranian Bacillus sp”. 16^th international &4^th national congress on biology, Iran. 2010
5. Marzieh Ghollasi, Khosro Khajeh, Ali Salimi. “Characterization of a mutagenised thermal- resistant α-amylase from Bacillus megaterium WHO”. 9^th National congress on Biochemistry and Biophysics, Iran.2009
6. Ali Salimi, Khosro Khajeh, Sirous Ghobadi, Marzieh Ghollasi and Jahan AliKhajeh. “Cloning, Sequencing, Expression and Characterization of a Ca²⁺-independent α-amylase from Bacillus sp. KRA2: emphasis on the identification of C-terminal propeptide”.10^th National congress on Biochemistry & 3^th internatiol congress on molecular biology Iran. 2009
7. Ali Salimi, Khosro Khajeh, Sirous Ghobadi, Marzieh Ghollasi and Jahan AliKhajeh. “Characterization of the C-terminal propeptide by cloning, sequencing and expression of a Ca2+ independent α-amylase from Bacillus sp. KRA2”. 14^th European Congress on Biotechnology. 2009
8. Marzieh Ghollasi, Khosro Khajeh, Hossein Naderi-Manesh. “Engineering of a Bacillus α-amylase whose stability is independent of calcium”. 14th European Congress on Biotechnology. 2009
9. Ali Salimi, Jahan AliKhajeh, Mohhamad Pazhang,  Marzieh Ghollasi, Sirous Ghobadi & Khosro Khajeh “Alpha Amylase from an Iranian Bacillus sp”. 15^th National & 3^th internatiol congress on biology, 2008
10. Maryam Ghanbari Safari, Khosro Khajeh, Marzieh Ghollasi, Hossein Naderi-manesh. “A novel extracellular α-amylase from a new isolated Bacillus sp. WHO: cloning, purification and biochemical characterization”. 10^th national Genetics Conference. 2008
11. Shekufeh Zareian, Khosro Khajeh, Marzieh Ghollasi, Bijan Ranjbar. “Purification and characterization of a novel pullulanase which converts pullulan and starch to glucose and maltose”. 33^rd FEBS Congress & 11^th IUBMB Conference. 2008
12. Maryam Ghanbari Safari, Khosro Khajeh, Marzieh Ghollasi, Hossein Naderi-manesh. “A novel extracellular α-amylase from a new isolated Bacillus sp. WHO: cloning, purification and biochemical characterization” 33^rd FEBS Congress & 11^th IUBMB Conference 2008
13. Marzieh Ghollasi, Khosro Khajeh, Nasrin mollania, Shekufeh Zareian. “Isolation, purification and biochemical analysis of a new pullulanase from  Geobacillus stearothermophilus”. The 3 rd Symposium on the Alpha-Amylase Family 2008
14. Shekufeh Zareian, Khosro Khajeh, Reza H.Sajedi, Bijan Ranjbar & Marzieh Ghollasi. “Screening, Isolation, Purification and characterization of pullulanase from a thermophilic strain of an hot spring”. 9^th national & 2^th international congress on biochemistry and molecular biology. 2007
15. Nasrin mollania, Khosro Khajeh, Mehdi Naderi-manesh, Marzieh Ghollasi. “Isolation, purification, biochemical characterization and cloning of a new extracellular α-amylase from Geobacillus thermodenitroficans LH₈ which is insensitive to phytic acid”. The 3 rd Symposium on the Alpha-Amylase Family 2007
16. Shekufeh Zareian, Marzieh Ghollasi, Khosro Khajeh, Saman Hosseinkhani, Bijan Ranjbar.” Isolation, purification and biochemical analysis of a new pullulanase from Geobacillus stearothermophilus”. The 3 rd Symposium on the Alpha-Amylase Family 2007.
17. M. Ghollasi, B. Farzami, S.Z. Bathaie and B.L.Rad. “Enzymatic- Like Properties of dsDNA and Its Comparison with ssDNA”. 6^th national congress on biochemistry & biophysic, Iran. 2005

Teaching Experiences

§  Enzymology (for M.Sc students of Biotechnology)

§  Radiation biology (for B.Sc students of cellular and molecular biology)

§  Biochemistry (for B.Sc students of biology)

§  Biochemistry of proteins and nucleic acids (for M.Sc students of Biochemistry)